同时检测水产品中醋酸甲羟孕酮和己烯雌酚残留的试剂盒

A kit for the simultaneous detection of medroxyprogesterone acetate and diethylstilbestrol in aquatic products

Abstract

The invention belongs to the field of bio-engineering techniques, and relates to a suspension array kit for the simultaneous detection of medroxyprogesterone acetate and diethylstilbestrol in aquatic products. The kit comprises monoclonal antibodies against medroxyprogesterone acetate and diethylstilbestrol. Using the suspension array for the simultaneous detection of medroxyprogesterone acetate and diethylstilbestrol of the kit comprises the steps of: pretreatment of a to-be-detected sample and detection of the sample by using the kit. The kit of the invention can simultaneously detect two indicators of medroxyprogesterone acetate and diethylstilbestrol residues, and has strong specificity, high sensitivity and good security; wherein the minimum detection limits of both medroxyprogesterone acetate and diethylstilbestrol are 0.4ng/ml. By using the suspension array method of the kit for the simultaneous detection of medroxyprogesterone acetate and diethylstilbestrol, the operation is simple and rapid, the result determination is accurate and reliable, the cost is low, and the investment is few. The kit of the present invention can be widely used in daily monitoring of veterinary drug residues in aquatic products.
本发明属于生物工程技术领域,涉及一种同时检测水产品中醋酸甲羟孕酮和己烯雌酚的液相芯片的试剂盒。该试剂盒包括针对醋酸甲羟孕酮和己烯雌酚的单克隆抗体。利用该试剂盒同时检测水产品中醋酸甲羟孕酮和己烯雌酚的液相芯片的步骤包括:前处理待测样品和用试剂盒检测样品。本发明的试剂盒可以同时检测醋酸甲羟孕酮和己烯雌酚两种残留指标,特异性强,敏感性高,安全性好,醋酸甲羟孕酮和己烯雌酚的最低检出限均为0.4ng/mL。利用该试剂盒同时检测水产品中醋酸甲羟孕酮和己烯雌酚的液相芯片的方法,操作简便快速,结果判定准确、可靠,成本低,投资少。本发明可以广泛应用于水产品兽药残留的日常监控。

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Cited By (1)

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    CN-103884837-AJune 25, 2014中国农业科学院上海兽医研究所Method for detecting nitrofuran metabolites in animal-derived food through liquid-phase chip