一种双水相体系分离富集牡丹花色苷的方法

Method for separating and enriching peony anthocyanin by using double-water-phase system

Abstract

本发明属于天然产物有效成分的分离纯化领域,特别公开了一种双水相体系分离富集牡丹花色苷的方法。本发明将牡丹鲜花低温烘干粉碎,加入由乙醇和硫酸铵构成的双水相体系,并添加蔗糖和有机酸作为花色苷的稳定剂,冰水浴条件下进行磁力搅拌,离心得上相、下相以及牡丹花残渣,进一步对残渣和下相进行多次萃取,然后合并上相进行醇沉除杂和萃取除杂,真空浓缩得牡丹花色苷。本发明采用双水相体系条件温和,提取时间短,有机相易回收,提取成本低,易于工业化放大生产。
The invention belongs to the field of separation and purification of natural product effective components, and particularly discloses a method for separating and enriching peony anthocyanin by using a double-water-phase system. The method comprises the following steps: drying and pulverizing fresh peony flowers at low temperature, adding a double-water-phase system composed of ethanol and ammonium sulfate, adding sucrose and organic acid as stabilizers of anthocyanin, magnetically stirring under ice water bath conditions, centrifugating to obtain an upper phase, a lower phase and peony residues, extracting the residues and lower phase many times, merging with the upper phase, carrying out impurity removal by alcohol precipitation and extraction, and carrying out vacuum concentration to obtain the peony anthocyanin. By adopting the double-water-phase system, the method has the advantages of mild conditions, short extraction time, recoverable organic phase and low extraction cost, and can easily implement industrialized scale-up production.

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Cited By (2)

    Publication numberPublication dateAssigneeTitle
    CN-104803960-AJuly 29, 2015云南民族大学Method for extracting and separating procyanidine of hawthorn fruit through low-temperature aqueous two phase cooling
    CN-104803960-BSeptember 22, 2017云南民族大学一种低温双水相冷却提取分离山楂肉原花青素的方法